If you are Sorting on the Cytopeia Influx High speed Sorter

Cytopeia Influx High Speed Sorter

Sample Cell concentration- 5-10 million cells per ml in 12x75mm

Polypropelene tubes. Cells can be in regular media with 5%FCS or less.

Tubes for collection of sorted cells- 12x75mm tubes, and 15ml

centrifuge tubes. Collection tubes should some have media in them (approximately 1ml for 12x75mm tubes, 2-3 ml for 15ml tubes.

Media in the collection tubes can have more than 5%FCS.

How many cells should you prepare?- The Influx Sorter can process 40-60 million cells per hour for primary cells at system pressures of 50PSI. Larger cells and “sticky” cell samples may require significantly lower data rates.

Always put your samples through a 40-70um mesh.

Bring additional collection tubes, pippetes, pippeters.

Bring additional collection media in a 50ml centrifuge tube.

Always Bring control cells, unstained sample(s) as well as relevant single fluorescence samples for compensation.

We do not have any tissue culture facilities.

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		Cytopeia Influx High Speed Sorter Sample Cell concentration- 5-10 million cells per ml in 12x75mm Polypropelene tubes. Cells can be in regular media with 5%FCS or less. Tubes for collection of sorted cells- 12x75mm tubes, and 15ml centrifuge tubes. Collection tubes should some have media in them (approximately 1ml for 12x75mm tubes, 2-3 ml for 15ml tubes. Media in the collection tubes can have more than 5%FCS. How many cells should you prepare?- The Influx Sorter can process 40-60 million cells per hour for primary cells at system pressures of 50PSI. Larger cells and “sticky” cell samples may require significantly lower data rates. Always put your samples through a 40-70um mesh. Always put your samples through a 40-70um mesh. Bring additional collection tubes, pippetes, pippeters. Bring additional collection media in a 50ml centrifuge tube. Always Bring control cells, unstained sample(s) as well as relevant single fluorescence samples for compensation. We do not have any tissue culture facilities.