PostSorts
   
The Flow Facility new BD Biosciences LSR II analyzer
   
       

The LSR II is equipped with 3 lasers , 488nm, 405nm, and 633nm. It can measure up to
8 fluorescent  parameters. For data acquisition and display it uses DIVA 6.
LSR II specifications.
488nm Laser(Blue Laser)        PMT Detector          Wv                Fluorochrome
                                                        A                  780 /60nm       PE_Cy7
                                                        B                  695 /40nm       PerCP-Cy5.5, PE-Cy5
                                                        C                  575/26nm        PE
                                                        D                  530/30nm        FITC, GFP

405 nm Laser (Violet Laser)           A                   525/50nm         AmCyan
                                                        B                   450/50nm         Pacific Blue,CFP

633nm Laser (Red Laser)               A                   780/60nm        APC-Cy7
                                                        B                   660/20nm        APC

For data acquisition the LSR II uses Diva 6.
For a tutorial please visit the link below.

http://www.bdbiosciences.com/immunocytometry_systems/support/training/online/diva5_course/

For more information on the LSR II:

http://www.bdbiosciences.com/pdfs/whitePapers/23-8641-00.pdf
http://www.bdbiosciences.com/pdfs/brochures/SJ-0142-00.pdf